Oral Health and Dental Management

Histomorphometric analysis for GMSCs in healthy versus inflamed and hyperplastic gingival tissue

3^rd Euro Congress and Expo on Dental & Oral Health

June 16-18, 2015 Alicante, Spain

Al Bahrawy M, Carrion J, GamalA, Iacono V and Ghaffar K

Posters-Accepted Abstracts: Oral Health Dent Manag

Abstract:

Objective: To detect Gingival mesenchymal stem cells (GMSCs) in tissue samples from healthy versus inflamed (chronic periodontitis) and hyperplastic (drug induced) using histomorphometric analysis. Methods: Twenty six tissue samples from different subjects of patients in periodontics department of Stony Brook school of dental medicine, whom been divided to three different groups: Healthy (gingival tissues of crown lengthening and distal wedge), Inflamed (gingival collars of apical repositioning flaps), Hyperplastic (gingival tissue of gingivectomy surgeries). Stem cells were detected using different markers namely Oct 3 / 4, CD 105, CD 90, Nucleostamin. Cells were counted in 5 different fields and mean was calculated. Results: Using ANOVA comparing the results of the three groups, no significant difference was found between chronic periodontitis samples and healthy, while statistical significant difference was found between hyperplastic samples and both other groups. Conclusion: GMSCs are found in healthy as well as inflamed gingival tissues with no statistically significant difference, while drug induced hyperplastic gingical tissue show significant difference in number of stem cells, which indicated that drug induced hyperplasia may recruit and/or stimulate the division of stem cells.